Rejection of impassable substrates by Yersinia type III secretion machines. Academic Article

abstract

• Type III machines of pathogenic Yersinia spp. transport Yop proteins across the bacterial envelope into host cells. Translational fusions of yopE to the dihydrofolate reductase gene (dhfr) or the beta-galactosidase gene (lacZ) generate hybrid proteins that block type III injection of Yop proteins into host cells, consistent with the canonical view that impassable DHFR and LacZ hybrids jam secretion machines. Mutations in repressors of posttranscriptional gene regulation, Yersinia enterocolitica yscM1 and yscM2 as well as Yersinia pestis lcrQ, relieve the YopE-DHFR-imposed blockade and restore type III injection into host cells. Genetic suppression of the type III blockade does not, however, promote YopE-DHFR secretion. A model is proposed whereby rejection of YopE-DHFR from the secretion pathway inhibits type III gene expression.

authors

• Sorg, Joseph

published proceedings

• J Bacteriol

author list (cited authors)

• Sorg, J. A., Miller, N. C., Marketon, M. M., & Schneewind, O.

citation count

• 28

complete list of authors

• Sorg, Joseph A||Miller, Nathan C||Marketon, Melanie M||Schneewind, Olaf

publication date

• October 2005

publisher

• American Society for Microbiology  Publisher

published in

• Journal of Bacteriology  Journal

keywords

• Bacterial Outer Membrane Proteins
• Bacterial Proteins
• Beta-Galactosidase
• Bodily Secretions
• Gene Expression Regulation, Bacterial
• Hela Cells
• Humans
• Lac Operon
• Recombinant Fusion Proteins
• Substrate Specificity
• Tetrahydrofolate Dehydrogenase
• Trans-Activators
• Virulence
• Yersinia Enterocolitica
• Yersinia Infections
• Yersinia Pestis

Digital Object Identifier (DOI)

• 10.1128/JB.187.20.7090-7102.2005

start page

• 7090

end page

• 7102

volume

• 187

issue

• 20

URL

• http://dx.doi.org/10.1128/jb.187.20.7090-7102.2005