Castro, Juan C. (2009-12). Fluorescein and Rosamine Derivatives as Donors/Acceptors for "Through-bond" Energy Transfer Cassettes. Master's Thesis. Thesis uri icon

abstract

  • A series of fluorescein and rosamine derivatives have been prepared and their spectroscopical properties analyzed to determine their usefulness as donor and/or acceptors in "through-bond" energy transfer systems. Such new systems have been tailored to possess higher quantum yields, increased water solubility and higher pH independence. Some of these compounds have also been designed with handles for bioconjugation for use in intracellular imaging. The syntheses of most of these compounds have been optimized to afford higher yields in a multigram scale. Single molecule studies on a fluorescein/rosamine cassette are also reported.
  • A series of fluorescein and rosamine derivatives have been prepared and their
    spectroscopical properties analyzed to determine their usefulness as donor and/or
    acceptors in "through-bond" energy transfer systems.
    Such new systems have been tailored to possess higher quantum yields, increased
    water solubility and higher pH independence. Some of these compounds have also been
    designed with handles for bioconjugation for use in intracellular imaging.
    The syntheses of most of these compounds have been optimized to afford higher
    yields in a multigram scale.
    Single molecule studies on a fluorescein/rosamine cassette are also reported.

publication date

  • December 2009