HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF TRISACCHARIDES ON AMINE-BONDED SILICA COLUMNS
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abstract
Thirteen different trisaccharides (3-O--cellobiosyl-d-glucose, cellotriose, 3-O--isomaltosyl-d-glucose, isomaltotriose, isopanose, 1-kestose, 4-O--laminaribiosyl-d-glucose, laminaritriose, maltotriose, melezitose, panose, raffinose, and xylotriose) were subjected to high-performance liquid chromatography on prepacked amino-bonded silica columns, using acetonitrile-water eluents. As with mono- and disaccharides, replacement of glucose by xylose or fructose caused shorter retention times, while its replacement by galactose led to delayed elution. Retention times of trisaccharides containing only glucose were shortest when only (1 3) bonds were present, and increased with increasing numbers of (1 4) and especially (1 6) bonds. When a trisaccharide contained two different types of bonds, retention times were roughly halfway between those of the two trisaccharides having only one type. 1985.
