Evaluation of three techniques (follicular aspiration, follicular aspiration and flushing and slicing of the ovaries) for recovery of equine oocytes from excised ovaries
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The objective of this study was to compare three different methods of oocyte recovery from equine ovaries obtained from an abattoir. The techniques used were a) aspiration of follicles, b) aspiration plus flushing of the follicles and c) slicing of the ovaries in a grid pattern. The number of oocytes recovered from each of the three methods was different (P<0.05). From 235 ovaries processed by the aspiration technique, a mean of .94 ± .5 oocytes was recovered per ovary. From 711 ovaries processed by the aspiration-flushing system a mean of 1.69 ± .46 oocytes per ovary was recovered and from 89 ovaries processed by the slicing technique a mean of 3.55 ± .45 oocytes perovary was collected. The effect of the system on the mean time spent recovering each oocyte was not significant. The type of system utilized had a significant effect (P<0.05) on the quality of oocytes. The mean number of grade 4 oocytes (completely or partially denuded) recovered with the slicing technique was greater than the mean number of grade 4 oocytes recovered by the aspiration-flushing technique. © 1993, William E. Jones. All rights reserved.
author list (cited authors)
Vazquez, J. C., Moreno, J. F., Hanneman, R., Evans, J. W., & Kraemer, D. C.