Immunologic effects of low levels of ochratoxin A in ovo: utilization of a chicken embryo model. Academic Article uri icon


  • Ochratoxin A (OA) was administered to 13-day-old chicken embryos via the chorioallantoic membrane. The 7-day LD50 value (day 20 incubation) of OA was calculated at 7.9 micrograms of OA. Ochratoxin-treated embryos (2.5 micrograms) had slight but significant changes in numbers of immunoglobulin-bearing cells in the bursa but not in the spleen. Chicks hatched from in ovo-treated eggs were challenged with 9 X 10(4) colony-forming units (CFU) of beta-hemolytic Escherichia coli (O1:K1) at 7 days of age via the thoracic air sac. Lesion scores of OA-treated chicks were equal to or less severe than those of controls. Hatchmates of the above chicks were vaccinated with a homologous killed E. coli bacterin (O1:K1) at both 2 and 4 weeks of age and challenged with 10(4) CFU of E. coli at 7 weeks. Post-challenge lesions were present in three vaccinated untreated controls and no OA-treated chicks. We conclude that although in ovo exposure to OA may marginally suppress immunoglobulin-bearing cells of bursa, chicks hatched from OA-treated eggs respond as well as controls to an antigen and resist infection by a virulent organism.

published proceedings

  • Avian Dis

altmetric score

  • 3

author list (cited authors)

  • Harvey, R. B., Kubena, L. F., Naqi, S. A., Gyimah, J. E., Corrier, D. E., Panigrahy, B., & Phillips, T. D.

citation count

  • 8

complete list of authors

  • Harvey, RB||Kubena, LF||Naqi, SA||Gyimah, JE||Corrier, DE||Panigrahy, B||Phillips, TD

publication date

  • October 1987