Survival of rough and smooth strains of Brucella abortus in bovine mammary gland macrophages. Academic Article uri icon


  • Chronic bovine brucellosis is characterized by persistent infection of the mammary gland. The interaction of live Brucella abortus with bovine mammary gland macrophages was studied in vitro. Opsonization of smooth B abortus strain 2308 and rough strain 45/20 was required for phagocytosis by mammary gland macrophages. When opsonized with specific antiserum, strains 2308 and 45/20 stimulated a considerable oxidative burst when phagocytized by mammary gland macrophages. Intracellular survival rates for strain 2308 were significantly higher than those for strain 45/20. After being phagocytized, B abortus localized in phagosomes and phagolysosomes of mammary gland macrophages.

published proceedings

  • Am J Vet Res

author list (cited authors)

  • Harmon, B. G., Adams, L. G., & Frey, M.

citation count

  • 79

complete list of authors

  • Harmon, BG||Adams, LG||Frey, M

publication date

  • July 1988