Fatty acid-binding protein activities in bovine muscle, liver and adipose tissue. Academic Article uri icon

abstract

  • Subcutaneous adipose tissue, sternomandibularis muscle and liver were obtained from steers immediately postmortem. Muscle strips and adipose tissue snips were incubated with 0.75 mM [1-14C]palmitate and 5 mM glucose. Muscle strips esterified palmitate at the rate of 2.5 nmol/min per gram tissue, which was 30% of the rate observed for adipose tissue. Fatty acid-binding protein activity was measured in 104,000 x g supernatant fractions of liver, muscle and adipose tissue homogenates. Muscle and adipose tissue fractions bound 840 and 140 pmol [1-14C]palmitoyl-CoA per gram tissue, respectively. Fatty acid-binding protein activity was greater in adipose tissue than in muscle when data were expressed per milligram protein (35 vs. 13 pmol palmitoyl-CoA bound per milligram of soluble protein, respectively). Fatty acid binding-protein activity was correlated with the rate of palmitate esterification within each tissue. Liver contained the highest fatty acid-binding protein activity (13,000 pmol palmitoyl-CoA bound per gram tissue and 215 pmol palmitoyl-CoA bound per milligrams soluble protein).

author list (cited authors)

  • Smith, S. B., Ekeren, P. A., & Sanders, J. O.

citation count

  • 11

publication date

  • November 1985