Agrobacterium tumefaciens-Mediated Transformation of Rosa hybrida using the Green Fluorescent Protein (GFP) Gene Academic Article uri icon

abstract

  • Embryogenic calluses of Rosa hybrida cultivar Tineke were transformed with Agrobacterium tumefaciens strain LBA4404 containing the binary vector pBIN m-gfp5-ER into which the virE/virG genes had been inserted. Visualization of GFP-expressing cells enabled visual selection of dividing, embryogenic cell clusters that were transgenic. When the Agrobacterium strain with the bifunctional fusion marker containing additional virE/virG genes was used, the number of green fluorescent calluses increased. Transformation of the GFP-expressing rose plants was confirmed by Southern blot analysis.

published proceedings

  • Plant Cell, Tissue and Organ Culture

altmetric score

  • 3

author list (cited authors)

  • Kim, C. K., Chung, J. D., Park, S. H., Burrell, A. M., Kamo, K. K., & Byrne, D. H.

citation count

  • 33

complete list of authors

  • Kim, CK||Chung, JD||Park, SH||Burrell, AM||Kamo, KK||Byrne, DH

publication date

  • August 2004