Aflp marker development in rose for genetic mapping: Comparison of three restriction enzyme pairs; Developpement de marqueurs aflp chez le rosier pour la cartographie genetique: Comparaison de trois paires d'enzymes de restriction
Academic Article
Overview
Additional Document Info
View All
Overview
abstract
AFLP technology was applied to construct genetic linkage maps in rose for use in marker assisted selection. The mapping progeny was created by crossing a black spot resistant amphidiploid (86-7) with a susceptible tetraploid (82-1134). One F1 hybrid of this cross, 90-69, was open-pollinated to obtain 115 seedlings. AFLP markers were first used to identify and eliminate seedlings produced through cross-fertilization. The remaining progeny set of 52 F2 plants were used in developing AFLP markers with three combinations of restriction enzymes, EcoRI/MseI, KpnI/MseI. and PstI/MseI. With EcoRI/MseI, primers with three selective bases for each restriction enzyme (+3/+3) yielded an optimum number of bands per gel. However, with KpnI/MseI and PstI/MseI, primers with +2/+3 selective bases produced well-separated bands equivalent in number and dispersion to those produced by +3/+3 selective bases of EcoRI/MseI. One linkage map was constructed for each parent using only single-dose markers. The map of 82-1134 consists of 167 markers assigned to 14 linkage groups and covers more than 682 cM of the genome. The map of 86-7 consists of 171 markers assigned to 15 linkage groups and covers more than 902 cM of its genome. A gene controlling the presence of prickles on the petiole was located at the end of linkage group 7 on the map of 86-7. EcoRI/MseI generated nearly twice as many markers per run than PstI/MseI. Markers developed with three restriction enzyme combinations showed a mixed distribution throughout the maps. Compared to EcoRI/MseI and KpnI/MseI AFLP markers, PstI/MseI markers had a slightly greater distribution in both maps. Overall, PstI/MseI AFLP markers provided the highest percentage of mapped markers.