Influence of ovule perforation, plant growth regulators, andl-glutamine on in vitro growth of immature peach embryos
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Ovule perforation technique and media components (plant growth regulators and l-glutamine) were tested on in vitro growth of immature (<3 mm) embryos of "Springcrest" and "Earligrande" peaches. Ovule perforation was 2 to 4 times more effective in promoting embryo growth than leaving ovules intact. l-Glutamine (400 mgliter -1) promoted an increase in growth but could not be used with indole-acetic acid plus kinetin because an antagonistic effect on embryo growth occurred. The use of these exogenous plant growth regulators did not increase embryo growth over in vivo growth. 1993 Tissue Culture Association.