Pea chloroplast tRNA(Lys) (UUU) gene: transcription and analysis of an intron-containing gene. Academic Article

abstract

• The pea chloroplast trnK gene which encodes tRNA(Lys) (UUU) was sequenced. TrnK is located 210 bp upstream from the promoter of psbA and immediately downstream from the 3'-end of rbcL. The gene is transcribed from the same DNA strand as psbA and rbcL. A 2447 bp intron with class II features is located in the trnK anticodon loop. The intron contains a 506 amino acid open reading frame which could encode an RNA maturase. The primary transcript of trnK is 2.9 kb long; its 5'-end was identified as a site of transcription initiation by in vitro transcription experiments. The 5'-terminus is adjacent to DNA sequences previously identified as transcription promoter elements. The most abundant trnK transcript is 2.5 kb long with termini corresponding to the 5' and 3' ends of the trnK exons. Intron specific RNAs were not detected. This suggests that RNA processing which produces tRNA(Lys) leads to rapid degradation of intron sequences.

authors

• Mullet, John

published proceedings

• Photosynth Res

author list (cited authors)

• Boyer, S. K., & Mullet, J. E.

citation count

• 17

complete list of authors

• Boyer, SK||Mullet, JE

publication date

• July 1988

publisher

• Springer Nature  Publisher

published in

• Photosynthesis Research  Journal

keywords

• 31 Biological Sciences
• 3102 Bioinformatics And Computational Biology
• 3105 Genetics
• Genetics

PubMed Central ID

• 24429659

Digital Object Identifier (DOI)

• 10.1007/BF00047679

start page

• 7

end page

• 22

volume

• 17

issue

• 1-2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2Fbf00047679