Plastid DNA synthesis and nucleic acid-binding proteins in developing barley chloroplasts. Academic Article

abstract

• Activation of plastid DNA synthesis occurred early in barley leaf chloroplast development. High rates of DNA synthesis were observed in the leaf basal meristem of dark-grown plants where plastid transcription activity was low. DNA synthesis activity decreased in later stages of chloroplast development. Plastid nuclei acid-binding proteins were detected after lithium dodecylsulfate (LDS)-polyacrylamide gel separation and renaturation. One set of nucleic acid-binding proteins was associated with plastid nucleoids. A second set of nucleic acid-binding proteins co-sedimented with ribosomes and most probably corresponds to ribosomal proteins. Changes in the composition of the nucleic acid-binding proteins were characterized as a function of chloroplast development in dark-grown and illuminated barley plants.

authors

• Mullet, John

published proceedings

• J Photochem Photobiol B

author list (cited authors)

• Baumgartner, B. J., & Mullet, J. E.

citation count

• 8

complete list of authors

• Baumgartner, BJ||Mullet, JE

publication date

• January 1991

publisher

• Elsevier  Publisher

published in

• Journal of Photochemistry and Photobiology, B: Biology  Journal

keywords

• Chloroplasts
• DNA
• DNA Replication
• DNA-Binding Proteins
• Darkness
• Electrophoresis, Polyacrylamide Gel
• Hordeum
• Light
• Transcription, Genetic

Digital Object Identifier (DOI)

• 10.1016/1011-1344(91)80261-f

start page

• 203

end page

• 218

volume

• 11

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2F1011-1344%2891%2980261-f