Induction, collection, and partial characterization of induced respiratory macrophages of the turkey. Academic Article

abstract

• Respiratory macrophages (RM) of the turkey were elicited with a 1:4 (v/v) suspension of incomplete Freund's adjuvant in sterile phosphate-buffered saline injected directly into the abdominal air sacs. RM were purified by passage through a Ficoll-Hypaque gradient resulting in 95.7 +/- 5.9% purity and 94.8 +/- 12.3% viability. On days 7 and 9 postinjection, adequate numbers (7.15 +/- 5.47 X 10(6) macrophages per turkey) of RM for in vitro experiments were obtained. RM of the turkey demonstrated the ability to adhere to glass, phagocytize Zymosan A, and kill Escherichia coli in vitro.

authors

• Edwards, John

published proceedings

• Avian Dis

author list (cited authors)

• Ficken, M. D., Edwards, J. F., & Lay, J. C.

citation count

• 37

complete list of authors

• Ficken, MD||Edwards, JF||Lay, JC

publication date

• October 1986

publisher

• JSTOR  Publisher

published in

• AVIAN DISEASES  Journal

keywords

• Animals
• Female
• Lung
• Macrophage Activation
• Macrophages
• Male
• Turkeys

PubMed Central ID

• 3814014

Digital Object Identifier (DOI)

• 10.2307/1590582

start page

• 766

end page

• 771

volume

• 30

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.2307%2F1590582