Phenolics from mango (Mangifera indica L.) suppress growth in different cancer cells, targeting pro-apoptotic and cell cycle control proteins Conference Paper uri icon

abstract

  • Gallotannins are phenolics in mango which have shown anticancer activity. The objective was to evaluate the antiprolioferative activity of mango phenolics in cancer cells. The cultivars Haden (Hd) and Ataulfo (At) were selected based on phenolic content and antioxidant activity. The antiproliferative and proapoptotic activities of phenolics extracts were tested in human Molt4 leukemia, A549 lung, MDAMB231 breast, SW480 colon and LnCap prostate cancer cells. Additionally, gene expression was investigated by RTPCR and cell cycle kinetics by flow cytometry. Hd inhibited the growth of SW480, Molt4, LnCap, MDAMB231, and A549 cells (IC50 = 2, 2.5, 7, 8.3, and 10.5 mg gallic acid equiv./L, respectively) as determined by cell counting at 72h, with SW480 cells being most sensitive to the treatments. In general, At extracts were less effective than Hd. HPLCPDA analysis of extracts revealed that Hd and At mainly contained gallotannins, though Hd contained higher concentration of lower molecular weight phenolics. The growth inhibition of Hd polyphenolics (15mg/L) in MDAMB231 was associated with increased expression of proapoptotic caspase8 and Bax by 1.2 and 1.5 fold, respectively. Furthermore, Hd (up to 15mg/L) induced cell cycle arrest in the S and G2/M phase. Overall, mango phenolics may possess chemopreventive activities by targeting proapoptotic genes and inducing cell cycle arrest.Grant Funding SourceNational Mango Board

published proceedings

  • FASEB JOURNAL

author list (cited authors)

  • Bertoldi, M. C., Noratto, G. D., Talcott, S. T., & Mertens-Talcott, S. U.

citation count

  • 0

complete list of authors

  • Bertoldi, Michele Correa||Noratto, Giuliana D||Talcott, Steve Terel||Mertens‐Talcott, Susanne U

publication date

  • April 2009

publisher