Engineered Split-TET2 Enzyme for Inducible Epigenetic Remodeling. Academic Article

abstract

• The Ten-eleven translocation (TET) family of 5-methylcytosine (5mC) dioxygenases catalyze the conversion of 5mC into 5-hydroxymethylcytosine (5hmC) and further oxidized species to promote active DNA demethylation. Here we engineered a split-TET2 enzyme to enable temporal control of 5mC oxidation and subsequent remodeling of epigenetic states in mammalian cells. We further demonstrate the use of this chemically inducible system to dissect the correlation between DNA hydroxymethylation and chromatin accessibility in the mammalian genome. This chemical-inducible epigenome remodeling tool will find broad use in interrogating cellular systems without altering the genetic code, as well as in probing the epigenotype-phenotype relations in various biological systems.

authors

• Huang, Yun

published proceedings

• J Am Chem Soc

altmetric score

• 10.75

author list (cited authors)

• Lee, M., Li, J., Liang, Y. i., Ma, G., Zhang, J., He, L., ... Huang, Y.

citation count

• 19

complete list of authors

• Lee, Minjung||Li, Jia||Liang, Yi||Ma, Guolin||Zhang, Jixiang||He, Lian||Liu, Yuliang||Li, Qian||Li, Minyong||Sun, Deqiang||Zhou, Yubin||Huang, Yun

publication date

• April 2017

publisher

• American Chemical Society (ACS)  Publisher

published in

• Journal of the American Chemical Society  Journal

keywords

• 5-Methylcytosine
• DNA-Binding Proteins
• Dioxygenases
• Epigenesis, Genetic
• Genetic Code
• HEK293 Cells
• Hela Cells
• Humans
• Models, Genetic
• Protein Engineering
• Proto-Oncogene Proteins

Digital Object Identifier (DOI)

• 10.1021/jacs.7b01459

URI

• https://hdl.handle.net/1969.1/169601

start page

• 4659

end page

• 4662

volume

• 139

issue

• 13

URL

• http://dx.doi.org/10.1021/jacs.7b01459