TET1‐Mediated Oxidation of 5‐Formylcytosine (5fC) to 5‐Carboxycytosine (5caC) in RNA
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It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.
author list (cited authors)
Basanta‐Sanchez, M., Wang, R., Liu, Z., Ye, X., Li, M., Shi, X., ... Sheng, J.