TET1-Mediated Oxidation of 5-Formylcytosine (5fC) to 5-Carboxycytosine (5caC) in RNA.
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It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.
author list (cited authors)
Basanta-Sanchez, M., Wang, R., Liu, Z., Ye, X., Li, M., Shi, X., ... Sheng, J
complete list of authors
Basanta-Sanchez, Maria||Wang, Rui||Liu, Zhenzhen||Ye, Xiaohan||Li, Minyong||Shi, Xiaodong||Agris, Paul F||Zhou, Yubin||Huang, Yun||Sheng, Jia