TET1-Mediated Oxidation of 5-Formylcytosine (5fC) to 5-Carboxycytosine (5caC) in RNA.

abstract

• It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.

altmetric score

• 1

author list (cited authors)

• Basanta-Sanchez, M., Wang, R., Liu, Z., Ye, X., Li, M., Shi, X., ... Sheng, J.

citation count

• 30

complete list of authors

• Basanta-Sanchez, Maria||Wang, Rui||Liu, Zhenzhen||Ye, Xiaohan||Li, Minyong||Shi, Xiaodong||Agris, Paul F||Zhou, Yubin||Huang, Yun||Sheng, Jia

publisher

• Wiley  Publisher

published in

• ChemBioChem  Journal

keywords

• 5-Methylcytosine
• 5-carboxycytidine
• 5-formylcytidine
• 5-methylcytidine
• Base Sequence
• Biocatalysis
• Catalytic Domain
• Chromatography, High Pressure Liquid
• Cytosine
• Humans
• Mixed Function Oxygenases
• Oxidation-Reduction
• Proto-Oncogene Proteins
• RNA
• Recombinant Proteins
• Tandem Mass Spectrometry
• Tet Enzyme

PubMed Central ID

• 27805801

Digital Object Identifier (DOI)

• 10.1002/cbic.201600328

start page

• 72

end page

• 76

volume

• 18

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1002%2Fcbic.201600328