TET1‐Mediated Oxidation of 5‐Formylcytosine (5fC) to 5‐Carboxycytosine (5caC) in RNA Academic Article uri icon


  • It was recently revealed that 5-methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), implying the potential regulatory roles of this post-transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5-carboxycytidine (5caC) by the catalytic domain of mammalian ten-eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single-stranded, double-stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.

altmetric score

  • 1

author list (cited authors)

  • Basanta‐Sanchez, M., Wang, R., Liu, Z., Ye, X., Li, M., Shi, X., ... Sheng, J.

citation count

  • 20

publication date

  • November 2016