The use of fungal in vitro systems for studying translational regulation. Book

abstract

• The use of cell-free systems enables biochemical determination of factors and mechanisms contributing to translational processes. The preparation and use of cell-free translation systems from the fungi Saccharomyces cerevisiae and Neurospora crassa are described. Examples provided illustrate the use of these systems, in conjunction with luciferase assays, [(35)S]Met incorporation, and primer-extension inhibition (toeprint) analyses, to assess the translational effects of upstream open reading frames and premature termination codons.

authors

• Sachs, Matthew

author list (cited authors)

• Wu, C., Amrani, N., Jacobson, A., & Sachs, M. S.

citation count

• 36

complete list of authors

• Wu, Cheng||Amrani, Nadia||Jacobson, Allan||Sachs, Matthew S

publication date

• January 2007

publisher

• Elsevier  Publisher

keywords

• Cell-Free System
• Cycloheximide
• DNA Primers
• Gene Expression Regulation, Fungal
• Micrococcal Nuclease
• Neurospora Crassa
• Open Reading Frames
• Peptide Chain Termination, Translational
• Peptide Termination Factors
• Protein Biosynthesis
• Ribosomes
• Saccharomyces Cerevisiae
• Saccharomyces Cerevisiae Proteins

PubMed Central ID

• 17913625

Digital Object Identifier (DOI)

• 10.1016/S0076-6879(07)29010-X

International Standard Book Number (ISBN) 13

• 978-0-12-374191-2

start page

• 203

end page

• 225

volume

• 429

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0076-6879%2807%2929010-x