Extracting DNA from submerged pine wood. Academic Article

abstract

• A DNA extraction protocol for submerged pine logs was developed with the following properties: (i) high molecular weight DNA, (ii) PCR amplification of chloroplast and nuclear sequences, and (iii) high sequence homology to voucher pine specimens. The DNA extraction protocol was modified from a cetyltrimehtylammonium bromide (CTAB) protocol by adding stringent electrophoretic purification, proteinase K, RNAse, polyvinyl pyrrolidone (PVP), and Gene Releaser. Chloroplast rbcL (ribulose-1,5-bisphosphate carboxylase) could be amplified. Nuclear ribosomal sequences had >95% homology to Pinus taeda and Pinus palustris. Microsatellite polymorphism for PtTX2082 matched 2 of 14 known P. taeda alleles. Our results show DNA analysis for submerged conifer wood is feasible.

authors

• Reynolds, Mollie

published proceedings

• Genome

altmetric score

• 3

author list (cited authors)

• Reynolds, M. M., & Williams, C. G

citation count

• 15

complete list of authors

• Reynolds, M Megan||Williams, Claire G

publication date

• October 2004

publisher

• Canadian Science Publishing  Publisher

published in

• Genome  Journal

keywords

• Cell Nucleus
• Cetrimonium
• Cetrimonium Compounds
• Chloroplasts
• DNA, Plant
• Endopeptidase K
• Microsatellite Repeats
• Pinus
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Povidone
• Ribonucleases
• Ribosomes
• Ribulose-Bisphosphate Carboxylase
• Wood

PubMed Central ID

• 15499414

Digital Object Identifier (DOI)

• 10.1139/g04-045

start page

• 994

end page

• 997

volume

• 47

issue

• 5

URL

• http%3A%2F%2Fdx.doi.org%2F10.1139%2Fg04-045