Surface-enhanced Raman spectroscopy for the in vitro and ex vivo detection of excitatory amino acids
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Traditional methods for the detection of excitatory amino acids, which have been linked to secondary injury following head trauma, can be excessively time consuming clinically. A near real-time measurement system could provide clinical information in anticipation of pharmaceutical intervention for head injured patients. Our studies have shown that Surface-Enhanced Raman Spectroscopy (SERS) with silver colloids has the ability to measure physiological concentrations of in vitro excitatory amino acids using short scan times. Employing a damage model for ischemia, preliminary ex vivo rat extracellular brain fluid analysis shows an intriguing correlation between SERS spectral features and expected Glutamate concentration fluctuations following head injuries.