The assignment of PRKCI to bovine chromosome 1q34 -> q36 by FISH suggests a new assignment to human chromosome 3 Academic Article uri icon

abstract

  • Two bovine BAC clones were identified by PCR as containing the bovine gene PRKCI. Both clones were assigned by FISH to bands q34-->q36 on BTA1. The sequence information derived from genomic DNA and from both clones was identical and showed a high degree of homology to human PRKCI (HSAXq21.3, 95.5% homology), and mouse Prkcl (MMU3, 13.8 cM, 87.6% homology) and rat Prkcl (88.8% homology). This assignment could suggest a disruption of the synteny conservation of mammalian X-linked genes, but most likely suggests a misassignment of this gene to the human X.

altmetric score

  • 3

author list (cited authors)

  • De Donato, M., Gallagher, D. S., Davis, S. K., Stelly, D. M., & Taylor, J. F.

citation count

  • 3

publication date

  • December 2001

keywords

  • Animals
  • Cattle
  • Chromosomes, Human, Pair 3
  • Humans
  • In Situ Hybridization, Fluorescence
  • Isoenzymes
  • Mice
  • Physical Chromosome Mapping
  • Protein Kinase C
  • Rats
  • Reproducibility Of Results
  • Sequence Homology, Nucleic Acid
  • Synteny
  • X Chromosome