Transduction of volume change in pH-sensitive hydrogels with resonance energy transfer
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abstract
A technique of optical transduction using fluorescence energy transfer (RET), to measure small volume change in gel-swelling response to environmental changes, for the determination of pH was demonstrated. A radiometric fluorescent sensor for pH measurement and high sensitivity and composition of biocompatible materials, changed hydrogel volume and energy transfer between donor and acceptor, in response to changing ionization of dual-labeled chitosan chains due to environmental pH. The fluorescence spectra for microsphere suspensions at different pH showed that the peak-intensity ratio decreased as pH of phosphate-buffered saline solution is decreased from 7.22 to 3.4. Fluorescent tags are advantageous to transduction and swelling because microspheres fabricated without covalent crosslinking are more porous showing that polymer dissolution occurs during pH testing. The increased ion concentration causes shrinkage in microstructures and the ratio of pH sensitivity to initial pH increased.
