Local oxygen level is density dependent in microchannel culture Conference Paper

abstract

• We present a measurement method of oxygen levels around cells cultured in a microchannel. Micro-scale silicate beads immobilised with Pt(II) octaethylporphine were used to measure the oxygen levels. Measurement of oxygen level was achieved by seeding beads near cells and observating the fluorescence emitted from the beads with a standard microscope. We demonstrated that the oxygen level approached physiologic levels as cell density increased to 400 cells/mm2. 2008 CBMS.

authors

• McShane, Michael

published proceedings

• 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

author list (cited authors)

• Hayashi, K., Berthier, E., Warrick, J., McShane, M. J., & Beebe, D. J.

complete list of authors

• Hayashi, K||Berthier, E||Warrick, J||McShane, MJ||Beebe, DJ

publication date

• January 2008

start page

• 1196

end page

• 1198