DEVELOPMENT OF A FIELD-PRACTICAL ASSAY FOR WATER-SOLVATED CHLOROPHENOLS
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A field-practical assay for chlorophenols (CPs) was developed and the accuracy, specificity and sensitivity were defined. The assay was capable of detecting these ubiquitous toxic compounds in water and has the potential for being used as a rapid, inexpensive screening test for potable water. The solvated target molecules were labeled with the fluorescent tag, 5-dimethylaminonaphthalene-1-sulfonyl chloride, dansyl chloride (DsCl). Extraction of the fluorescent complex allowed for the removal of co-contaminants. Detection of the dansylated CPs was based on the visualization of induced bluish-yellow fluorescence. The accuracy, specificity and sensitivity of the assay were determined by: (i) testing diverse organic and inorganic contaminants (listed in the 1992 US Primary Drinking Water Standards), (ii) testing aqueous CP solutions of known concentration, and iii) testing potable water samples collected from six Texas cities. 1995.