Vanadium‐induced inhibition of renal na+, k+‐adenosinetriphosphatase in the chicken after chronic dietary exposure Academic Article uri icon

abstract

  • Recent work has shown that V accumulates in the kidney and is a potent inhibitor of Na+, K+-adenosinetriphosphatase (ATPase) in vitro. Thus, as a nutritionally required element, V may regulate cation transport. The effect of chronic intake of the metal on Na+, K+-ATPase in vivo has not been reported. In this study laying strain chickens were fed calcium orthovanadate for 15 mo from d 1 of age at levels of 0, 25, 50, and 100 ppm in the diet. Whole tissue homogenates and 13,000 X g fractions were analyzed for ATPase activities. Concentrations of V producing 50% inhibition of Na+, K+-ATPase activity ranged from 1.0 X 10(-5) M in liver to 1.8 X 10(-6) M in kidney, which was the most sensitive tissue tested in vitro. Mg2+ -ATPase was more resistant to V than Na+, K+-ATPase. Studies in vivo suggested a V-dependent inhibition of renal Na+, K+-ATPase. Correlation of enzyme specific activity and levels of V in kidneys suggested V-ATPase mediated alteration in renal function.

altmetric score

  • 3

author list (cited authors)

  • Phillips, T. D., Nechay, B. R., Neldon, S. L., Kubena, L. F., Heidelbaugh, N. D., Shepherd, E. C., Stein, A. F., & Hayes, A. W.

citation count

  • 8

complete list of authors

  • Phillips, TD||Nechay, BR||Neldon, SL||Kubena, LF||Heidelbaugh, ND||Shepherd, EC||Stein, AF||Hayes, AW

publication date

  • April 1982