Effect of gibberellin biosynthesis inhibitors on shoot regeneration from hypocotyl explants of Albizzia julibrissin. Academic Article

abstract

• Hypocotyl explants of Albizzia julibrissin were placed on Gamborg's B5 medium supplemented with various levels of paclobutrazol, uniconazole, prohexadione calcium, or GA3. Callus formation was evident within one week after placement of the explants on the culture media. Green nodule-like structures protruded from the distal end of the explants within 10 days and developed into shoots within a month. These shoots readily formed adventitious roots when placed on fresh culture medium. All three of the gibberellin biosynthesis inhibitors increased shoot formation compared to the control. The number of shoots per explants was increased 107, 79, and 168% by 0.3-0.4 M paclobutrazol, uniconazole, and prohexadione calcium, respectively. In contrast to the gibberellin biosynthesis inhibitors, GA3 decreased shoot formation. These results indicate that modification of gibberellin status can have a strong impact on the number of shoots formed.

authors

• Davis, Tim

published proceedings

• Plant Cell Rep

author list (cited authors)

• Sankhla, D., Davis, T. D., & Sankhla, N.

citation count

• 14

complete list of authors

• Sankhla, D||Davis, TD||Sankhla, N

publication date

• December 1993

publisher

• Springer Nature  Publisher

published in

• Plant Cell Reports  Journal

keywords

• Adventitious Shoot Formation
• Gibberellic Acid
• Paclobutrazol
• Prohexadione Calcium
• Tissue Culture
• Uniconazole

Digital Object Identifier (DOI)

• 10.1007/BF00235303

start page

• 115

end page

• 118

volume

• 13

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2Fbf00235303