Verification of cooking endpoint temperatures in beef by immunoassay of lactate dehydrogenase isozyme 5 Academic Article uri icon


  • Ground beef Semimembranosus muscle was inoculated with Escherichia coli, and aliquots were left raw or cooked to 1 of 24 time/temperature combinations. Samples were analyzed by aerobic plate counts for surviving bacteria, total extractable protein, and immunoblot quantification of lactate dehydrogenase isozyme 5 (LDH5). Bacterial thermal death curves exhibited a consistent log decline with increasing temperature and cooking times at a given temperature, which were paralleled by decreases in LDH5 at 61C and above. Total protein exhibited a linear time/temperature decline. Immunologic analysis of LDH5 may be suitable for development into a rapid assay method for the verification in beef of cooking endpoint temperatures from 61C to 69C.

published proceedings


author list (cited authors)

  • Miller, D. R., Keeton, J. T., Acuff, G. R., & Prochaska, J. F.

citation count

  • 4

complete list of authors

  • Miller, DR||Keeton, JT||Acuff, GR||Prochaska, JF

publication date

  • August 2003