Fast macro-scale transmission imaging of microvascular networks using KESM. Academic Article uri icon

abstract

  • Accurate microvascular morphometric information has significant implications in several fields, including the quantification of angiogenesis in cancer research, understanding the immune response for neural prosthetics, and predicting the nature of blood flow as it relates to stroke. We report imaging of the whole mouse brain microvascular system at resolutions sufficient to perform accurate morphometry. Imaging was performed using Knife-Edge Scanning Microscopy (KESM) and is the first example of this technique that can be directly applied to clinical research. We are able to achieve 0.7m resolution laterally with 1m depth resolution using serial sectioning. No alignment was necessary and contrast was sufficient to allow segmentation and measurement of vessels.

published proceedings

  • Biomed Opt Express

altmetric score

  • 1

author list (cited authors)

  • Mayerich, D., Kwon, J., Sung, C., Abbott, L., Keyser, J., & Choe, Y.

citation count

  • 34

complete list of authors

  • Mayerich, David||Kwon, Jaerock||Sung, Chul||Abbott, Louise||Keyser, John||Choe, Yoonsuck

publication date

  • October 2011