EVALUATION OF DIFFERENT SULFUR-COMPOUNDS IN THE DIET OF JUVENILE SUNSHINE BASS (MORONE CHRYSOPS FEMALE-XM-SAXATILIS MALE)
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A semipurified basal diet with 35% crude protein, 0.38% methionine and 0.13% cystine was supplemented with different sulfur compounds to evaluate their efficacy in satisfying the total sulfur amino acid requirement of sunshine bass (Morone chrysops M. saxatilis ). A control diet was supplemented with l-methionine to meet the total sulfur amino acid requirement of sunshine bass, and test diets were supplemented with either dl-methionine, dl-methionine hydroxy analog (MHA), N-acetyl-dl-methionine, taurine or glutathione on an equal-sulfur basis with the control diet. Fingerlings of sunshine bass (mean initial wt SD = 11.3 0.1 g) were reared in a brackish water recirculating system, and triplicate groups of fish were fed the different diets twice daily. After 8 weeks of feeding, the greatest weight gain as well as best feed and protein utilization values was observed among fish fed diets containing l-methionine, dl-methionine and N-acetyl-dl-methionine. Glutathione and MHA were about 75% as effective in promoting growth compared with l-methionine, while taurine was totally ineffective. Fish fed glutathione had a significantly greater hepatosomatic index (HSI; liver weight 100/body weight) and lower intraperitoneal fat ratio (IPF ratio; intraperitoneal fat weight 100/body weight). Plasma methionine level was highest in fish fed dl-methionine. Highest whole-body protein levels were found in fish fed l-methionine, N-acetyl-dl-methionine, and dl-methionine, while the lowest level was in fish fed glutathione. Based on these results, dl-methionine and N-acetyl-dl-methionine were most effective relative to l-methionine in satisfying the sulfur amino acid requirement of sunshine bass. 1995.
