Adaptation of nucleic acid extraction methods for animal feeds Academic Article uri icon

abstract

  • Animal feeds contain a wide variety of microorganisms that may cause animal and human health problems. It would be desirable to rapidly detect as many of these organisms as possible. The objective of this study was the evaluation of nucleic acid extraction protocols to determine an optimal sample processing strategy for DNA isolation from different animal feeds. DNA extraction methods were applied to selected animal feeds to determine which could consistently provide DNA visible by gel electrophoresis. Comparable recovery of DNA extraction protocols was achieved after 20 g of each feed samples were mixed with 0.2% peptone containing 0.01% Triton X-100. For 9 different feeds visible DNA could be consistently detected for each feed indicating that a detergent-based dilution step prior to DNA extraction recovered sufficient DNA regardless of the feed matrix.

published proceedings

  • JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY

author list (cited authors)

  • Maciorowski, K. G., Pillai, S. D., & Ricke, S. C.

citation count

  • 5

complete list of authors

  • Maciorowski, KG||Pillai, SD||Ricke, SC

publication date

  • December 2001

publisher