Presence of bacteriophages in animal feed as indicators of fecal contamination. Academic Article

abstract

• The objectives of this study were to determine if indigenous male specific and somatic bacteriophages could be detected in animal feeds and if isolated phages contained RNA or DNA. Seven fresh feeds, 2 fresh feed ingredients, 7 stored feeds, 2 stored feed ingredients, and 8 samples of poultry diets suspected to contain Salmonella spp. were enriched and spot plated for indigenous phages using Escherichia coli Famp and CN-13 as hosts. Bacteriophage numbers were below detection without enrichment, but both male specific and somatic coliphages were detected in all animal feeds, feed ingredients, and poultry diets after 16 h of enrichment, even after the samples had been stored for 14 months of storage at -20 C. Five out of 9 fresh feeds and 7 out of 8 stored feeds contained RNA somatic phages.

authors

• Pillai, Suresh

published proceedings

• J Environ Sci Health B

author list (cited authors)

• Maciorowski, K. G., Pillai, S. D., & Ricke, S. C.

citation count

• 20

complete list of authors

• Maciorowski, KG||Pillai, SD||Ricke, SC

publication date

• September 2001

publisher

• Taylor & Francis  Publisher

published in

• Journal of Environmental Science and Health Part B: Pesticides, Food Contaminants, and Agricultural Wastes  Journal

keywords

• Animal Feed
• Animals
• Bacteriophages
• Colony Count, Microbial
• Enterobacteriaceae
• Feces
• Food Contamination
• Food Handling
• Food Microbiology
• Male
• Poultry
• Salmonella
• Time Factors

Digital Object Identifier (DOI)

• 10.1081/PFC-100106196

start page

• 699

end page

• 708

volume

• 36

issue

• 5

URL

• http%3A%2F%2Fdx.doi.org%2F10.1081%2Fpfc-100106196