Efficacy of an immuno-magnetic separation system for recovering Cryptosporidium oocysts from soils

abstract

• Studies were conducted to determine the efficacy of the commercially available immuno-magnetic system by Dynal to recover C. parvum oocysts from silty clay, sandy clay loam and clay soils. Each soil type was spiked with known numbers of oocysts and their recovery using percoll-sucrose gradient centrifugation in combination with immuno-magnetic separation system was evaluated. The recoveries varied significantly. The silty clay loam soil had the highest recovery ranging between 91% and 26%, while the sandy clay loam had the lowest recovery ranging between 30% and 2%. The results indicate that though the Dynal IMS system is capable of recovering oocysts from soils, the recovery efficiencies can vary significantly.

authors

• Pillai, Suresh

published proceedings

• JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY

author list (cited authors)

• McElroy, W., Cabello, E., & Pillai, S. D.

citation count

• 6

complete list of authors

• McElroy, W||Cabello, E||Pillai, SD

publication date

• August 2001

publisher

• Wiley  Publisher

published in

• Journal of Rapid Methods and Automation in Microbiology  Journal

Digital Object Identifier (DOI)

• 10.1111/j.1745-4581.2001.tb00230.x

start page

• 63

end page

• 70

volume

• 9

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1745-4581.2001.tb00230.x