ISOLATION OF NUCLEI FROM THE UNICELLULAR ALGA OLISTHODISCUS-LUTEUS (CHLOROMONADOPHYCEAE) Academic Article uri icon

abstract

  • ABSTRACTMethods are described for the isolation of nuclei from the marine chloromonad Olisthodiscus luteus. For each of the three alternative methods, the ratios to DNA of RNA, acidsoluble and acidinsoluble protein are 0. 17, 1.1 and 2.0, respectively. The nuclear preparations are free of whole cell and organelle contamination, although some membranes are present. The yield is 6580% based on the recovery of DNA in the nuclear pellet. The nuclei are in DNAdependent RNA synthesis.

published proceedings

  • JOURNAL OF PHYCOLOGY

author list (cited authors)

  • RIZZO, P. J., & BURGHARDT, R. C.

citation count

  • 5

publication date

  • September 1983

publisher