Potentiometric vs amperometric sensing of glycerol using glycerol dehydrogenase immobilized via layer-by-layer self-assembly Academic Article uri icon

abstract

  • 2014, Springer-Verlag Wien. An electrochemical biosensor for glycerol was obtained by using a novel concatenation of molecules to immobilize glycerol dehydrogenase (GlDH) on a gold electrode via layer-by-layer (LBL) self-assembly. The surface of the enzyme electrodes was characterized by cyclic voltammetry and scanning electron microscopy which confirmed the attachment of enzyme on the gold electrode with the assistance of the tethering molecules. The biosensor was assessed for its potentiometric and amperometric response to glycerol in the presence of the enzyme stimulants, ammonium sulfate and manganese chloride. The electrodes demonstrated good selectivity and reproducibility, with a amperometric response at a working voltage of 1.3V in the 0.001 to 1M glycerol concentration range, a 12.07A M1 sensitivity, and a 6.8M lower limit of detection. The average diffusion coefficient of glycerol is 8.63 106 cm2s1 as determined by chronoamperometry.

published proceedings

  • MICROCHIMICA ACTA

author list (cited authors)

  • Mahadevan, A., Gunawardena, D. A., Karthikeyan, R., & Fernando, S.

citation count

  • 7

complete list of authors

  • Mahadevan, Aishwarya||Gunawardena, Duminda A||Karthikeyan, Raghupathy||Fernando, Sandun

publication date

  • February 2015