Regulation of hypoxanthine transport in Neurospora crassa. Academic Article uri icon


  • Hypoxanthine uptake and hypoxanthine phosphoribosyltransferase activity (EC were determined in germinated conidia from the adenine auxotrophic strains ad-1 and ad-8 and the double mutant strain ad-1 ad-8. The mutant strain ad-1 appears to lack aminoimidazolecarboximide ribonucleotide formyltransferase (EC or inosine 5'monophosphate cyclohydrolase (EC activities, or both, whereas the ad-8 strain lacks adenylosuccinate synthase activity (EC Normal (or wild-type) hypoxanthine transport capacity was found to the ad-1 conidia, whereas the ad-8 strains failed to take up any hypoxanthine. The double mutant strains showed intermediate transport capacities. Similar results were obtained for hypoxanthine phosphoribosyl-transferase activity assayed in germinated conidia. The ad-1 strain showed greatest activity, the ad-8 strain showed the least activity, and the double mutant strain showed intermediate activity levels. Ion-exchange chromatography of the growth media revealed that in the presence of NH+/4, the ad-8 strain excreted hypoxanthine or inosine, the ad-1 strain did not excrete any purines, and the ad-1 ad-8 double mutant strain excreted uric acid. In the absence of NH+/4, none of the strains excreted any detectable purine compounds.

published proceedings

  • J Bacteriol

author list (cited authors)

  • Sabina, R. L., Magill, J. M., & Magill, C. W.

citation count

  • 9

complete list of authors

  • Sabina, RL||Magill, JM||Magill, CW

publication date

  • November 1976