Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase. Academic Article

abstract

• The Escherichia coli gene murZ, encoding the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase, has been cloned and sequenced. Identified by screening an E. coli genomic library for clones that conferred phosphomycin resistance, murZ encoded a 419-amino-acid polypeptide and was mapped to 69.3 min on the E. coli chromosome. MurZ protein was purified to near homogeneity and found to have the expected UDP-N-acetylglucosamine enolpyruvyl transferase activity. Sequence analysis of the predicted product revealed 44% identity to OrfR from Bacillus subtilis (K. Trach, J.W. Chapman, P. Piggot, D. LeCoq, and J.A. Hoch, J. Bacteriol. 170:4194-4208, 1988), suggesting that orfR may also encode a UDP-N-acetylglucosamine enolpyruvyl transferase enzyme. MurZ is also homologous to the aromatic amino acid biosynthetic enzyme enolpyruvyl shikimate phosphate synthase, the other enzyme known to catalyze an enolpyruvyl transfer.

authors

• Siegele, Deborah

altmetric score

• 6

author list (cited authors)

• Marquardt, J. L., Siegele, D. A., Kolter, R., & Walsh, C. T.

citation count

• 114

complete list of authors

• Marquardt, JL||Siegele, DA||Kolter, R||Walsh, CT

publication date

• January 1992

publisher

• American Society for Microbiology  Publisher

published in

• Journal of Bacteriology  Journal

keywords

• Alkyl And Aryl Transferases
• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• Chromosome Mapping
• Cloning, Molecular
• DNA, Bacterial
• Escherichia Coli
• Genes, Bacterial
• Molecular Sequence Data
• Molecular Weight
• Mutagenesis, Insertional
• Transferases

Digital Object Identifier (DOI)

• 10.1128/jb.174.17.5748-5752.1992

start page

• 5748

end page

• 5752

volume

• 174

issue

• 17

URL

• http%3A%2F%2Fdx.doi.org%2F10.1128%2Fjb.174.17.5748-5752.1992