Discovery of nuclear and plastid microsatellites, and other key genomic information, in the rare endemic plant (Caulanthus amplexicaulis var. barbarae) using minimal 454 pyrosequencing Academic Article uri icon

abstract

  • Next-generation Roche 454 pyrosequencing was used to rapidly identify 149 novel nuclear microsatellite loci and five plastid microsatellites from non-enriched genomic DNA of the rare endemic Santa Barbara Jewelflower (Caulanthus amplexicaulis var. barbarae). Analysis of this dataset showed a high level of orthology to annotated genes of the model plant Arabidopsis thaliana, as well as low levels of repetitive DNA sequences (particularly transposon-like elements). Thus, a modest sequencing effort using the 454 (or equivalent long-read platform) is a cost-effective and highly informative first step in the development of molecular-genetic resources for a non-model organism. © Springer Science+Business Media B.V. 2011.

author list (cited authors)

  • Burrell, A. M., No, E., & Pepper, A. E.

citation count

  • 4

publication date

  • May 2011