Molecular cloning and functional expression of a serotonin receptor from the Southern cattle tick, Boophilus microplus (Acari: Ixodidae). Academic Article

abstract

• A full-length cDNA encoding a 5-hydroxytryptamine (5-HT) receptor from the Southern cattle tick, Boophilus microplus, was isolated using a strategy based on sequence homology among G protein-coupled receptors. The deduced amino acid sequence revealed highest identity with Drosophila melanogaster 5HT-dro2A (Z11489, 50.8%) and 5HT-dro2B (Z11490, 49.5%) receptors. The receptor was transiently expressed in mammalian HEK293 cells, and Western blot analysis showed the expected 43.3 kDa band. In these cells, application of 5-HT (10 microm) inhibited forskolin-induced cAMP synthesis by 26%. The results indicate that the tick receptor is an invertebrate 5-HT1-like receptor that couples to Galphai protein.

authors

• Pietrantonio, Patricia

published proceedings

• Insect Mol Biol

author list (cited authors)

• Chen, A., Holmes, S. P., & Pietrantonio, P. V.

citation count

• 29

complete list of authors

• Chen, A||Holmes, SP||Pietrantonio, PV

publication date

• February 2004

publisher

• Wiley  Publisher

published in

• INSECT MOLECULAR BIOLOGY  Journal

keywords

• Amino Acid Sequence
• Animals
• Base Sequence
• Blotting, Western
• Cells, Cultured
• Cyclic AMP
• DNA Primers
• DNA, Complementary
• Gene Expression
• Ixodidae
• Molecular Sequence Data
• Receptors, Serotonin
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Alignment
• Sequence Analysis, DNA
• Transfection

PubMed Central ID

• 14728666

Digital Object Identifier (DOI)

• 10.1111/j.1365-2583.2004.00457.x

start page

• 45

end page

• 54

volume

• 13

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1365-2583.2004.00457.x