Isolation of the V-ATPase A and c subunit cDNAs from mosquito midgut and Malpighian tubules. Academic Article uri icon


  • Using conserved amino acid sequences for the design of oligonucleotide primers, we isolated cDNA clones for two subunits of the V-ATPase from the midgut and Malpighian tubules of Aedes aegypti larvae. The 3.1 kb cDNA of the A subunit of the peripheral catalytic V1 sector codes for a protein of 68.6 kDa. The protein contains conserved motifs, including an ATP/GTP binding site, found in all other A subunits. Southern analysis using the A subunit as a probe suggests the presence of only a single copy of gene in the Aedes aegypti. The 0.85 kb cDNA of the c subunit of the membrane H+ conducting V0 sector codes for a protein of kDa. This protein has four transmembrane domains and contains a conserved glutamic acid that serves as the binding site for dicyclohexylcarbodiimide. Southern analysis using the c subunit as a probe suggests the presence of more than one related gene in the genome of Aedes aegypti. Pileup analysis of various A and c subunits shows that these subunits fall into distinct clusters, including one in which all arthropod proteins are clustered.

published proceedings

  • Arch Insect Biochem Physiol

altmetric score

  • 6

author list (cited authors)

  • Gill, S. S., Chu, P. B., Smethurst, P., Pietrantonio, P. V., & Ross, L. S.

citation count

  • 18

complete list of authors

  • Gill, SS||Chu, PB||Smethurst, P||Pietrantonio, PV||Ross, LS

publication date

  • January 1998