Cloning and transcriptional expression of a leucokinin-like peptide receptor from the southern cattle tick, Boophilus microplus (Acari: Ixodidae). Academic Article

abstract

• Leucokinins are invertebrate neuropeptides that exhibit myotropic and diuretic activity. Only one leucokinin-like peptide receptor is known, the lymnokinin receptor from the mollusc Lymnaea stagnalis. A cDNA encoding a leucokinin-like peptide receptor was cloned from the Southern cattle tick, Boophilus microplus, a pest of cattle world-wide. This is the first neuropeptide receptor known from the Acari and the second known in the subfamily of leucokinin-like peptide G-protein-coupled receptors. The deduced amino acid sequence exhibits 40% identity to the lymnokinin receptor. The receptor transcript is present in all tick life stages as determined by semiquantitative reverse transcription polymerase chain reaction. We also propose that the sequence AAF50775.1 from the Drosophila melanogaster genome (CG10626) encodes the first identified insect leucokinin receptor.

authors

• Pietrantonio, Patricia

published proceedings

• Insect Mol Biol

altmetric score

• 3

author list (cited authors)

• Holmes, S. P., He, H., Chen, A. C., lvie, G. W., & Pietrantonio, P. V.

citation count

• 47

complete list of authors

• Holmes, SP||He, H||Chen, AC||lvie, GW||Pietrantonio, PV

publication date

• October 2000

publisher

• Wiley  Publisher

published in

• INSECT MOLECULAR BIOLOGY  Journal

keywords

• Amino Acid Sequence
• Animals
• Arthropod Proteins
• Base Sequence
• Cloning, Molecular
• Gene Expression
• Molecular Sequence Data
• Molecular Weight
• Open Reading Frames
• Polymerase Chain Reaction
• Receptors, Neuropeptide
• Reverse Transcriptase Polymerase Chain Reaction
• Ticks

PubMed Central ID

• 11029664

Digital Object Identifier (DOI)

• 10.1046/j.1365-2583.2000.00208.x

start page

• 457

end page

• 465

volume

• 9

issue

• 5

URL

• http%3A%2F%2Fdx.doi.org%2F10.1046%2Fj.1365-2583.2000.00208.x