Cloning of an aquaporin-like cDNA and in situ hybridization in adults of the mosquito Aedes aegypti (Diptera: Culicidae). Academic Article

abstract

• A cDNA encoding a putative water channel protein, aquaporin, was cloned from a cDNA library of Aedes aegypti Malpighian tubules. The cDNA encodes a 26.11 kDa protein similar to insect aquaporins from Haematobia irritans exigua (Diptera) and Cicadella viridis (Homoptera), and to mammalian aquaporin 4. Localization of the messenger RNA (mRNA) was performed by in situ hybridization of Malpighian tubules and analysed by fluorescence and confocal microscopy. The mRNA was localized in tracheolar cells associated with the Malpighian tubules. No signal was detected in the Malpighian tubule epithelium. The molecular mechanisms for water movement between tissues and tracheoles are not yet elucidated in insects. Our results suggest a model to explain fluid movements in tracheoles during insect respiration.

authors

• Pietrantonio, Patricia

published proceedings

• Insect Mol Biol

author list (cited authors)

• Pietrantonio, P. V., Jagge, C., Keeley, L. L., & Ross, L. S.

citation count

• 58

complete list of authors

• Pietrantonio, PV||Jagge, C||Keeley, LL||Ross, LS

publication date

• August 2000

publisher

• Wiley  Publisher

published in

• INSECT MOLECULAR BIOLOGY  Journal

keywords

• Aedes
• Amino Acid Sequence
• Animals
• Aquaporins
• Base Sequence
• Cloning, Molecular
• DNA, Complementary
• Female
• Humans
• In Situ Hybridization
• Molecular Sequence Data
• Sequence Homology, Amino Acid

PubMed Central ID

• 10971718

Digital Object Identifier (DOI)

• 10.1046/j.1365-2583.2000.00201.x

start page

• 407

end page

• 418

volume

• 9

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1046%2Fj.1365-2583.2000.00201.x