SEPARATION OF CAROTENOIDS IN TURF BERMUDA GRASSES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY Academic Article

abstract

• A simple, rapid high-performance liquid chromatographic method has been developed to separate and quantify the major carotenoids present in turf Bermuda grasses (Cynodon dactylon) without isomerization and oxidation. A reversed-phase isocratic solvent system of water-acetonitrile-chloroform (2:83:15) provided a clear separation of neoxanthin, violaxanthin, lutein and -carotene. Separation occurred within 10 min with detection at 470 nm and a sensitivity at 0.01 a.u.f.s. -apo-8-Carotenal was used as an internal standard to quantify all the compounds. This method was found to be very reproducible with coefficients of variation less than 3% in 5 sample analyses. The amount of neoxanthin, violaxanthin, lutein and -carotene in turf Bermuda grasses was 106, 127, 535 and 253 /g, respectively. 1987.

authors

• Bailey, Christopher

published proceedings

• JOURNAL OF CHROMATOGRAPHY

author list (cited authors)

• CHEN, B. H., & BAILEY, C. A.

citation count

• 6

complete list of authors

• CHEN, BH||BAILEY, CA

publication date

• January 1987

publisher

• Elsevier  Publisher

published in

• Journal of Chromatography A  Journal

keywords

• 34 Chemical Sciences
• 3401 Analytical Chemistry

Digital Object Identifier (DOI)

• 10.1016/S0021-9673(01)94226-8

start page

• 297

end page

• 304

volume

• 393

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0021-9673%2801%2994226-8