A new procedure to prepare slides of metaphase chromosomes of roses Academic Article uri icon


  • To our knowledge, there has been no published technique to produce consistently high-quality slides of somatic chromosomes of roses (Rosa sp.). Therefore, various pretreatments, fixatives, digestions, stains, and maceration and squashing methods were tested to identify a procedure to produce clear, well-spread chromosomes from shoot tips. The best results were obtained after pretreatment in a mixture of 0.1% colchicine and 0.001 m 8-hydroxyquinoline for 4 h, and fixation in 2 acetone: 1 acetic acid (v/v) with 2% (w/v) polyvinylpyrrolidone. The darkest-stained chromosomes were obtained with carbol-fuchsin staining of air-dried cell suspensions that had been spread in 3 ethanol: 1 acetic acid (v/v).

published proceedings


author list (cited authors)

  • Ma, Y., IslamFaridi, M. N., Crane, C. F., Stelly, D. M., Price, H. J., & Byrne, D. H.

citation count

  • 21

complete list of authors

  • Ma, Y||IslamFaridi, MN||Crane, CF||Stelly, DM||Price, HJ||Byrne, DH

publication date

  • September 1996