Polarimetric detection of glucose in biological based fluids
Additional Document Info
The use of polarimetry in the investigation of chiral molecules has been researched for over a century. However, it has not been until recently that the sensitivity and accuracy of this technology has improved enough to be applied to the quantification of the low optically active metabolite concentrations seen within the body. The long term goal of this research is the development of a polarimetric detection system with enough sensitivity to estimate both in vivo and in vitro metabolite concentrations, such as glucose, in the presence of other confounders (i.e. protein, ascorbic acid, and lactic acid). In this study, a robust polarimeter utilizing digital closed-loop control was designed and constructed that can effectively measure millidegree rotations of plane-polarized light within biological media. In vitro experiments were conducted using a 1 cm path length sample cell in both glucose doped cell culture and bovine aqueous humor media with analyte concentrations on the order of those seen in the body. A high degree of linearity between the measured signal and glucose concentration is seen during calibration of both the cell culture and aqueous humor experiments with correlation coefficients of 0.9995 and 0.9912, respectively. In addition, validation of the obtained calibration models yielded standard errors of prediction of 8.469 and 20.25 mg/dl for each media, respectively. Overall, we feel the conducted experiments are a logical step to furthering the development of using polarimetry in the detection of optically active metabolites, and our results indicate that accurate detection of glucose in the presence of additional confounders can be accomplished in both cell culture and aqueous humor media.
name of conference
Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology