Feasibility of an on line fluorescence-based optical sensor for oxygen monitoring in cell culture media Conference Paper uri icon


  • An oxygen sensor is under development to be part of a battery of optically based sensors for future cell culture experiments to be conducted by the National Aeronautics and Space Administration (NASA). This fluorescence-based sensor is intended to be indwelling within a self-contained cell culture apparatus designed for long-term experiments typical of those performed in the space shuttle or international space station. Our probe takes advantage of a well-documented characteristic of oxygen to quench most fluorophores. In this example, a species of ruthenium dye which is known to exhibit a considerable reduction in luminescence via oxygen quenching is immobilized in a poly(ethylene glycol) (PEG) hydrogel and immobilized in aqueous media. External to the vessel that holds the sensor and the cell culture media, a single fiber delivers LED excitation light while other fibers collect the emission light and return it to a detector. The sensor has been shown in initial trials to exhibit a high correlation to secondary oxygen sensors and retains good reproducibility across a range of oxygen concentrations that are typical for cell culture experiments (0-9.1 ppm O2at 35). Sensor hydrogels retain their physical shape and sensitivity to oxygen over a period of at least days.

name of conference

  • Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring II

published proceedings


author list (cited authors)

  • O'Neal, D. P., Meledeo, M. A., Pishko, M. V., & Cote, G. L.

citation count

  • 2

complete list of authors

  • O'Neal, DP||Meledeo, MA||Pishko, MV||Cote, GL

editor list (cited editors)

  • Priezzhev, A. V., & Cote, G. L.

publication date

  • January 2002