Determination of cell culture medium components with overlapping near-IR absorbance peaks
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abstract
A noninvasive method to measure levels of important tissue culture nutrients and metabolic byproducts would provide the potential for computer control of the growth process, decreasing the amount of human attention necessary while improving efficiency and repeatability of experiments by precise maintenance of important parameters without contaminating the culture environment. Our approach to meeting this need is the development of optical sensors that extract chemical information based on the absorbance of near-infrared radiation. Near-infrared spectroscopy of the 2.0-2.5 m region was used to collect absorbance data of aqueous mixtures containing glucose, lactate, ammonia, glutamate, and glutamine, as well as cell culture media samples from a three-day growth period. Multivariate calibration was used with these data to prove that complicated spectra can yield reasonable prediction errors. Calibration models using different combinations of spectra from aqueous mixtures and culture media were built and evaluated for glucose, ammonia, and lactate. Prediction errors of 2%, 8%, and 15% were obtained for glucose, lactate, and ammonia, respectively.
