A fluorescence polarization based assay for glucose sensing Conference Paper

abstract

• A fluorescence polarization (FP) assay was developed to determine concentrations of glucose using concanavalin A (ConA) and fluorescently-labeled dextran. Predictive FP responses to glucose were elicited for different assay configurations using mathematical modeling and displayed herein. Using 4 kDa FITC-dextran, we predicted a change of 0.120 P units from 0 mg/dL glucose to 500 mg/dL. This shows the potential that a homogenous, reproducible FP assay can be engineered to measure glucose concentrations using tetrameric ConA and 4k kDa FITC-dextran. 2012 SPIE.

name of conference

• Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV

authors

• Cote, Gerard

published proceedings

• OPTICAL DIAGNOSTICS AND SENSING XII: TOWARD POINT-OF-CARE DIAGNOSTICS; AND DESIGN AND PERFORMANCE VALIDATION OF PHANTOMS USED IN CONJUNCTION WITH OPTICAL MEASUREMENT OF TISSUE IV

author list (cited authors)

• Cummins, B. M., & Cote, G. L.

citation count

• 0

complete list of authors

• Cummins, Brian M||Cote, Gerard L

editor list (cited editors)

• Nordstrom, R. J., & Coté, G. L.

publication date

• January 2012

publisher

• SPIE, the international society for optics and photonics  Publisher

published in

• Proceedings of SPIE  Journal

keywords

• Biosensors
• Competitive Binding
• Fluorescence Polarization
• Glucose Sensing

Digital Object Identifier (DOI)

• 10.1117/12.908630

International Standard Book Number (ISBN) 13

• 9780819488725

start page

• 82291c-82291c-7

volume

• 8229

URL

• http%3A%2F%2Fdx.doi.org%2F10.1117%2F12.908630