PEGylation of Concanavalin A to decrease nonspecific interactions in a fluorescent glucose sensor Conference Paper uri icon

abstract

  • The ability of people with diabetes to both monitor and regulate blood sugar levels is limited by the conventional "finger-pricktest" that provides intermittent, single point measurements. Toward the development of a continuous glucose monitoring (CGM) system, the lectin, Concanavalin A (ConA), has been utilized as a component in a Förster resonance energy transfer (FRET), competitive glucose binding assay. Recently, to avoid reversibility problems associated with ConA aggregation, a suitable competing ligand labeled with 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt (APTS) has been engineered. However, its ability to function as part of a glucose sensing assay is compromised due to the negative charge (at physiological pH) of native ConA that gives rise to non-specific binding with other ConA groups as well as with electrostatically charged assay-delivery carriers. To minimize these undesirable interactions, we have conjugated ConA with monomethoxy-poly(ethylene glycol) (mPEG) (i.e. "PEGylation"). In this preliminary research, fluorescently-labeled ConA was successfully PEGylated with mPEG- Nhydroxylsuccinimide(succinimidyl carbonate) (mPEG-NHS(SC)). The FRET response of APTS-labeled competing ligand (donor) conveyed an increase in the fluorescence intensity with increasing glucose concentrations. © 2014 SPIE.

author list (cited authors)

  • Abraham, A. A., Cummins, B. M., Locke, A. K., Grunlan, M. A., & Coté, G. L.

citation count

  • 0

publication date

  • February 2014

publisher