Immunological response to the Brucella abortus GroEL homolog. Academic Article

abstract

• Western blot (immunoblot) analysis of sera from cattle vaccinated with Brucella abortus S19 exhibit an elevated serologic response to Hsp62, the GroEL homolog (BaGroEL). Serologic screening of individual cows vaccinated with B. abortus S19 revealed no correlation between the immune response to BaGroEL and protection against a challenge with virulent organisms. The humoral immune response to BaGroEL was restricted to a region of the mature protein which mapped to amino acids 317 to 355 and may represent a useful diagnostic tool for monitoring exposure to B. abortus. Immunity to a challenge with virulent B. abortus S2308 was not observed in the BaGroEL vaccinated mouse model.

authors

• Adams, Leslie

published proceedings

• Infect Immun

author list (cited authors)

• Lin, J., Adams, L. G., & Ficht, T. A.

citation count

• 28

complete list of authors

• Lin, J||Adams, LG||Ficht, TA

publication date

• October 1996

publisher

• American Society for Microbiology  Publisher

published in

• Infection and Immunity  Journal

keywords

• Animals
• Antibodies, Bacterial
• Blotting, Western
• Brucella Abortus
• Cattle
• Chaperonin 60
• Female
• Mice
• Rabbits
• Vaccination

PubMed Central ID

• 8926120

Digital Object Identifier (DOI)

• 10.1128/iai.64.10.4396-4400.1996

URI

• https://hdl.handle.net/1969.1/179810

start page

• 4396

end page

• 4400

volume

• 64

issue

• 10

URL

• http://dx.doi.org/10.1128/iai.64.10.4396-4400.1996