Ratio-based decisions and the quantitative analysis of cDNA microarray images. Academic Article

abstract

• Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed. 1997 Society of Photo-Optical Instrumentation Engineers.

authors

• Dougherty, Edward

published proceedings

• J Biomed Opt

altmetric score

• 3

author list (cited authors)

• Chen, Y., Dougherty, E. R., & Bittner, M. L.

citation count

• 626

complete list of authors

• Chen, Y||Dougherty, ER||Bittner, ML

publication date

• October 1997

publisher

• SPIE, the international society for optics and photonics  Publisher

published in

• Journal of Biomedical Optics  Journal

PubMed Central ID

• 23014960

Digital Object Identifier (DOI)

• 10.1117/12.281504

start page

• 364

end page

• 374

volume

• 2

issue

• 4