Ratio-based decisions and the quantitative analysis of cDNA microarray images.
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Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed. 1997 Society of Photo-Optical Instrumentation Engineers.
author list (cited authors)
Chen, Y., Dougherty, E. R., & Bittner, M. L.
complete list of authors
Chen, Y||Dougherty, ER||Bittner, ML