Ratio-based decisions and the quantitative analysis of cDNA microarray images. Academic Article uri icon

abstract

  • Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed. 1997 Society of Photo-Optical Instrumentation Engineers.

published proceedings

  • J Biomed Opt

altmetric score

  • 3

author list (cited authors)

  • Chen, Y., Dougherty, E. R., & Bittner, M. L.

citation count

  • 612

complete list of authors

  • Chen, Y||Dougherty, ER||Bittner, ML

publication date

  • October 1997