Morphological slice-based counting of illuminated 3D bodies
Academic Article
Overview
Identity
Additional Document Info
Other
View All
Overview
abstract
This paper presents a simulation toolbox for counting illuminated 3D bodies. The current model is limited to illuminated balls but can be extended to other illuminated bodies. Upon simulation of a set of balls in space, horizontal slices are taken to provide a stack of 2D gray-scale images. Based on theses images, a morphological algorithm estimates the number of balls. Construction of the toolbox has been motivated by the need to count spots in FISH images to test for elevated gene copy numbers. The toolbox facilitates analysis of various algorithm parameters based on the distribution of the 3D bodies. These include the number of slices and various settings for the morphological filters composing the algorithm. 2000 SPIE 0277-786X/00/$15.00.